The Canonical pre-mRNA 3'-end Processing Project

Updated Jan. 2023

Most eukaryotic messenger RNA precursors (pre-mRNAs) must undergo extensive maturational processing, including 5'-end capping, splicing, and 3'-end cleavage and polyadenylation. The addition of a poly(A) tail is important for mRNA stability, and enhances mRNA transport to the cytoplasm and mRNA translation.

The 3'-end processing events include cleavage at a specific site in the pre-mRNA followed by the addition of the poly(A) tail. In mammals, the cleavage site is defined by an upstream AAUAAA motif and a downstream U or G/U-rich element.

A large number of protein factors have been identified that are crucial for pre-mRNA 3'-end processing. These proteins form several sub-complexes, such as the cleavage and polyadenylation specificity factor (CPSF) and the cleavage stimulation factor (CstF). CPSF contains 5 subunits, CPSF-30, -73, -100, -160, and Fip1, and CstF contains 3 subunits, Cstf-50, -64, and -77. CPSF-160 recognizes the upstream AAUAAA motif, and CstF-64 recognizes the downstream U- or G/U-rich element.

Major findings from this project

Publications from this project

Funding for this project

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